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T7513
Sigma
Thrombin from bovine plasma
lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)
| Synonym: | Factor IIa |
| CAS Number: | 9002-04-4 |
| Enzyme Commission (EC) Number: | 3.4.21.5 ( BRENDA | IUBMB ) |
| EC Number: | 232-648-7 |
| MDL number: | MFCD00166053 |
Description
| Analysis Note | Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K. |
| The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations. | |
| Application | Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. |
| Biochem/physiol Actions | Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B. |
| Other Notes | View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer. |
| Physical form | Lyophilized from saline sodium citrate buffer, pH 6.5 |
Properties
| form | lyophilized powder |
| storage temp. | −20°C |
Safety
| Safety Statements | 22-24/25 |
| WGK Germany | 2 |
| RTECS | XO8950000 |
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| Inhibitor | A9141, Antithrombin III from bovine plasma |
| D0879, Diisopropylfluorophosphate | |
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References
| reference | , R. Biggs, ed. Human Blood Coagulation, Haemostasis and Thrombosis 2nd ed., Philadelphia , (1976), 722 |
| Merck | Merck 13,9461 |
